solvent delivery systems pn1122 Search Results


hplc pump postnova analytics pn1122  (Postnova Analytics)
90
Postnova Analytics hplc pump postnova analytics pn1122
(A) 50-100 μg of OxlT was loaded onto a Shodex kw-803 <t>HPLC</t> column in the presence of 5% glycerol, 50 mM potassium MOPS, 100 mM potassium oxalate and 0.1% DDM (pH 7). The tracings show normalized outputs of detectors for measurement of A280 (UV, black), Light Scattering (LS, red) and Refractive Index (RI, blue). In a parallel trial in the same experiment and using the same buffer conditions, the instrument calibration constant K1 was obtained using a reference set of six protein standards of mass between 29 kDa and 141 kDa (see Experimental Procedures). (B) For illustrative purposes, using data from Part A, OxlT mass (red trace) was calculated for the region across the A280 trace at ca. 75% peak height. For this and other experiments, the mass values reported in Tables ​Tables11 and ​and22 were calculated at the peak of the A280 trace.
Hplc Pump Postnova Analytics Pn1122, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hplc pump postnova analytics pn1122 - by Bioz Stars, 2026-03
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hp-88 column  (Agilent technologies)
90
Agilent technologies hp-88 column
(A) 50-100 μg of OxlT was loaded onto a Shodex kw-803 <t>HPLC</t> column in the presence of 5% glycerol, 50 mM potassium MOPS, 100 mM potassium oxalate and 0.1% DDM (pH 7). The tracings show normalized outputs of detectors for measurement of A280 (UV, black), Light Scattering (LS, red) and Refractive Index (RI, blue). In a parallel trial in the same experiment and using the same buffer conditions, the instrument calibration constant K1 was obtained using a reference set of six protein standards of mass between 29 kDa and 141 kDa (see Experimental Procedures). (B) For illustrative purposes, using data from Part A, OxlT mass (red trace) was calculated for the region across the A280 trace at ca. 75% peak height. For this and other experiments, the mass values reported in Tables ​Tables11 and ​and22 were calculated at the peak of the A280 trace.
Hp 88 Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hp-88 column/product/Agilent technologies
Average 90 stars, based on 1 article reviews
hp-88 column - by Bioz Stars, 2026-03
90/100 stars
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sprague-dawley rats  (Dawley Inc)
90
Dawley Inc sprague-dawley rats
(A) 50-100 μg of OxlT was loaded onto a Shodex kw-803 <t>HPLC</t> column in the presence of 5% glycerol, 50 mM potassium MOPS, 100 mM potassium oxalate and 0.1% DDM (pH 7). The tracings show normalized outputs of detectors for measurement of A280 (UV, black), Light Scattering (LS, red) and Refractive Index (RI, blue). In a parallel trial in the same experiment and using the same buffer conditions, the instrument calibration constant K1 was obtained using a reference set of six protein standards of mass between 29 kDa and 141 kDa (see Experimental Procedures). (B) For illustrative purposes, using data from Part A, OxlT mass (red trace) was calculated for the region across the A280 trace at ca. 75% peak height. For this and other experiments, the mass values reported in Tables ​Tables11 and ​and22 were calculated at the peak of the A280 trace.
Sprague Dawley Rats, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sprague-dawley rats/product/Dawley Inc
Average 90 stars, based on 1 article reviews
sprague-dawley rats - by Bioz Stars, 2026-03
90/100 stars
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capillary column hp-88  (Agilent technologies)
90
Agilent technologies capillary column hp-88
(A) 50-100 μg of OxlT was loaded onto a Shodex kw-803 <t>HPLC</t> column in the presence of 5% glycerol, 50 mM potassium MOPS, 100 mM potassium oxalate and 0.1% DDM (pH 7). The tracings show normalized outputs of detectors for measurement of A280 (UV, black), Light Scattering (LS, red) and Refractive Index (RI, blue). In a parallel trial in the same experiment and using the same buffer conditions, the instrument calibration constant K1 was obtained using a reference set of six protein standards of mass between 29 kDa and 141 kDa (see Experimental Procedures). (B) For illustrative purposes, using data from Part A, OxlT mass (red trace) was calculated for the region across the A280 trace at ca. 75% peak height. For this and other experiments, the mass values reported in Tables ​Tables11 and ​and22 were calculated at the peak of the A280 trace.
Capillary Column Hp 88, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/capillary column hp-88/product/Agilent technologies
Average 90 stars, based on 1 article reviews
capillary column hp-88 - by Bioz Stars, 2026-03
90/100 stars
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af2000 focus system  (Postnova Analytics)
90
Postnova Analytics af2000 focus system
(A) 50-100 μg of OxlT was loaded onto a Shodex kw-803 <t>HPLC</t> column in the presence of 5% glycerol, 50 mM potassium MOPS, 100 mM potassium oxalate and 0.1% DDM (pH 7). The tracings show normalized outputs of detectors for measurement of A280 (UV, black), Light Scattering (LS, red) and Refractive Index (RI, blue). In a parallel trial in the same experiment and using the same buffer conditions, the instrument calibration constant K1 was obtained using a reference set of six protein standards of mass between 29 kDa and 141 kDa (see Experimental Procedures). (B) For illustrative purposes, using data from Part A, OxlT mass (red trace) was calculated for the region across the A280 trace at ca. 75% peak height. For this and other experiments, the mass values reported in Tables ​Tables11 and ​and22 were calculated at the peak of the A280 trace.
Af2000 Focus System, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/af2000 focus system/product/Postnova Analytics
Average 90 stars, based on 1 article reviews
af2000 focus system - by Bioz Stars, 2026-03
90/100 stars
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pn 5200 sample injector  (Postnova Analytics)
90
Postnova Analytics pn 5200 sample injector
(A) 50-100 μg of OxlT was loaded onto a Shodex kw-803 <t>HPLC</t> column in the presence of 5% glycerol, 50 mM potassium MOPS, 100 mM potassium oxalate and 0.1% DDM (pH 7). The tracings show normalized outputs of detectors for measurement of A280 (UV, black), Light Scattering (LS, red) and Refractive Index (RI, blue). In a parallel trial in the same experiment and using the same buffer conditions, the instrument calibration constant K1 was obtained using a reference set of six protein standards of mass between 29 kDa and 141 kDa (see Experimental Procedures). (B) For illustrative purposes, using data from Part A, OxlT mass (red trace) was calculated for the region across the A280 trace at ca. 75% peak height. For this and other experiments, the mass values reported in Tables ​Tables11 and ​and22 were calculated at the peak of the A280 trace.
Pn 5200 Sample Injector, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pn 5200 sample injector/product/Postnova Analytics
Average 90 stars, based on 1 article reviews
pn 5200 sample injector - by Bioz Stars, 2026-03
90/100 stars
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anti-protein tyrosine phosphatase non-receptor type 11 peptide 2  (Developmental Studies Hybridoma Bank)
N/A
PTPN11
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recombinant mouse pabpn1 protein  (Creative BioMart)
N/A
Recombinant Mouse PABPN1 full length or partial length protein was expressed http www creativebiomart net Recombinant Mouse PABPN1 Protein 444842 htm
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psicor ptpn11i2 plasmid 59613  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
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ppn112 plasmid 91639  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
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Image Search Results


(A) 50-100 μg of OxlT was loaded onto a Shodex kw-803 HPLC column in the presence of 5% glycerol, 50 mM potassium MOPS, 100 mM potassium oxalate and 0.1% DDM (pH 7). The tracings show normalized outputs of detectors for measurement of A280 (UV, black), Light Scattering (LS, red) and Refractive Index (RI, blue). In a parallel trial in the same experiment and using the same buffer conditions, the instrument calibration constant K1 was obtained using a reference set of six protein standards of mass between 29 kDa and 141 kDa (see Experimental Procedures). (B) For illustrative purposes, using data from Part A, OxlT mass (red trace) was calculated for the region across the A280 trace at ca. 75% peak height. For this and other experiments, the mass values reported in Tables ​Tables11 and ​and22 were calculated at the peak of the A280 trace.

Journal: Biochemistry

Article Title: Oligomeric State of the Oxalate Transporter, OxlT †

doi: 10.1021/bi201175y

Figure Lengend Snippet: (A) 50-100 μg of OxlT was loaded onto a Shodex kw-803 HPLC column in the presence of 5% glycerol, 50 mM potassium MOPS, 100 mM potassium oxalate and 0.1% DDM (pH 7). The tracings show normalized outputs of detectors for measurement of A280 (UV, black), Light Scattering (LS, red) and Refractive Index (RI, blue). In a parallel trial in the same experiment and using the same buffer conditions, the instrument calibration constant K1 was obtained using a reference set of six protein standards of mass between 29 kDa and 141 kDa (see Experimental Procedures). (B) For illustrative purposes, using data from Part A, OxlT mass (red trace) was calculated for the region across the A280 trace at ca. 75% peak height. For this and other experiments, the mass values reported in Tables ​Tables11 and ​and22 were calculated at the peak of the A280 trace.

Article Snippet: Molecular weight determination by TD-SEC-LS Analytical size exclusion chromatography was conducted using a Postnova Analytics PN1122 HPLC pump in conjunction with a Shodex KW-G guard column in line with a Shodex KW-803 size exclusion column.

Techniques: Refractive Index